Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7042 |
| PDBID: | 4TNW |
| Chains: | Uh_Q |
| Organism: | Caenorhabditis elegans, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1038/nature13669 |
| Antibody | |
| Antibody: | 3RHW Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Avermectin-sensitive glutamate-gated chloride channel GluCl alpha |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: U
Mutation: NULL
| >4TNW_U|Chain CA[auth F], F[auth G], G[auth H], H[auth I], I[auth J], S[auth U], T[auth V], U[auth W], V[auth X], W[auth Y]|Mouse monoclonal Fab fragment, heavy chain|Mus musculus (10090) EVQLQQSGPELVRPGASMKISCKASGYSFTGYTMNWVKQSHGKNLEWIGLINPYNGGTSYNQKFKGKATLTVDKSSSTAYMELLSLTSEDSAVYYCARDGDYYRYGRYFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: h
Mutation: NULL
| >4TNW_h|Chain AA[auth K], BA[auth L], DA[auth M], J[auth N], K[auth O], L[auth Z], M[auth f], X[auth g], Y[auth h], Z[auth i]|Mouse monoclonal Fab fragment, light chain|Mus musculus (10090) QAVVTQESALTTSPGETVTLTCRSSTGAVTTINFANWVQEKPDHLFTGLIGGINNRAPGVPARFSGSLIGDKAALTITGAQTEDEAIYFCALWYSNHWVFGGGTKLTVLGQPKSSPSVTLFPPSSEELETNKATLVCTITDFYPGVVTVDWKVDGTPVTQGMETTQPSKQSNNKYMASSYLTLTARAWERHSSYSCQVTHEGHTVEKSLSRADCS |
Antigen
Chain: Q
Mutation: NULL
| >4TNW_Q|Chain A, B, C, D, E, N[auth P], O[auth Q], P[auth R], Q[auth S], R[auth T]|Avermectin-sensitive glutamate-gated chloride channel GluCl alpha|Caenorhabditis elegans (6239) SDSKILAHLFTSGYDFRVRPPTDNGGPVVVSVNMLLRTISKIDVVNMEYSAQLTLRESWIDKRLSYGVKGDGQPDFVILTVGHQIWMPDTFFPNEKQAYKHTIDKPNVLIRIHNDGTVLYSVRISLVLSCPMYLQYYPMDVQQCSIDLASYAYTTKDIEYLWKEHSPLQLKVGLSSSLPSFQLTNTSTTYCTSVTNTGIYSCLRTTIQLKREFSFYLLQLYIPSCMLVIVSWVSFWFDRTAIPARVTLGVTTLLTMTAQSAGINSQLPPVSYIKAIDVWIGACMTFIFCALLEFALVNHIANAGTTEWNDISKRVDLISRALFPVLFFVFNILYWSRFGHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| U: THR33 LEU50 ASN52 ASN55 GLY56 GLY57 THR58 SER59 TYR103 ARG104 TYR105 ARG107 h: THR26 GLY27 THR31 ILE32 PHE34 ILE53 TRP93 SER95 ASN96 Q: ASN24 GLY25 GLY26 PRO27 VAL29 THR155 LYS156 GLU159 LEU161 THR189 TYR190 CYS191 THR192 SER193 VAL194 THR195 ASN196 GLY198 ILE199 ARG204 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
