Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 704 |
| PDBID: | 4CDG |
| Chains: | D_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.79 |
| Reference: | 10.1093/nar/gkv373 |
| Antibody | |
| Antibody: | 4CDG Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | BLOOM SYNDROME PROTEIN |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4CDG_D|Chain C, D|NANOBODY|LAMA GLAMA (9844) MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQAGGSLRLSCAASGIWFSINNMAWYRQTPGKQRERIAIITSAGTTNYVDSVKGRFTISRDDAKNTMYLQMNSLIPEDTAVYYCNLVADYDMGFQSFWGRGTQVTVSSHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >4CDG_B|Chain A, B|BLOOM SYNDROME PROTEIN|HOMO SAPIENS (9606) SMSRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGMSENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition D: ARG41 SER52 ILE53 ASN54 ASN55 TYR59 ARG69 ILE72 THR74 SER75 ALA76 THR78 ASN80 ARG93 ASP95 ALA96 LYS97 TYR101 GLN103 VAL120 ASP122 TYR123 ASP124 PHE127 SER129 B: TYR995 TYR1024 HIS1028 GLU1031 ILE1033 THR1110 MET1111 ASN1112 MET1113 ASP1116 SER1123 ALA1124 LYS1125 GLU1143 LYS1147 ILE1150 LEU1151 ASP1156 GLU1157 LEU1159 ILE1161 GLN1166 ALA1167 ILE1168 ALA1169 TYR1170 SER1195 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
