Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7028 |
| PDBID: | 4R0L |
| Chains: | AB_C |
| Organism: | Mus musculus, Canis lupus familiaris |
| Method: | XRD |
| Resolution (Å): | 3.30 |
| Reference: | 10.1097/TXD.0000000000000516 |
| Antibody | |
| Antibody: | 1C6 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T-cell costimulatory molecule CD28 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4R0L_A|Chain A, D[auth H]|Antibody 1C6 Heavy chain|Mus musculus (10090) SQIQLVQSGPELKKPGETVKISCKASGYTFTNYGMTWVKQAPRKGLKWMGWINTYTGRPTYADDFKGRFAFSLETSASTAYLQINNLKHEDTATYFCASLGEDFWGQGTTLTVSSHHHHHH |
Light Chain: B
Mutation: NULL
| >4R0L_B|Chain E[auth B], F[auth L]|Antibody 1C6 Light chain|Mus musculus (10090) MDIVMTQAAPSVPVTPGESVSISCRSTKSLLHSNGNTYLYWFLQRPGQSPQRLIYYMSNLASGVPDRFSGRGSGTDFTLRISRVEAEDAGVYYCMQSLEYPYTFGGGTKLEIKRLVP |
Antigen
Chain: C
Mutation: NULL
| >4R0L_C|Chain B[auth C], C[auth D]|T-cell costimulatory molecule CD28|Canis lupus familiaris (9615) SNKILVKQLPRLVVYNNEVNLSCKYTYNLFSKEFRASLYKGVDSAVEVCVVNGNYSHQPQFYSSTGFDCDGKLGNETVTFYLRNLFVNQTDIYFCKIEVMYPPPYIGNEKSNGTIIHVKSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR30 ASN31 TYR32 GLY33 TRP50 THR52 LEU96 GLY97 ASP99 PHE100 B: HIS27 SER28 ASN29 ASN31 TYR33 TYR35 ARG47 TYR50 TYR51 ASN54 SER57 TYR95 C: SER21 PHE29 GLU32 ASN51 GLY52 ASN53 TYR54 SER55 HIS56 GLN57 PRO58 GLN59 PHE60 TYR61 SER62 SER63 ASP69 LYS71 LEU72 GLY73 ASN74 GLU75 THR76 THR78 TYR80 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
