Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7021 |
| PDBID: | 4PIR |
| Chains: | I_C |
| Organism: | Mus musculus, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/nature13552 |
| Antibody | |
| Antibody: | 15 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 5-hydroxytryptamine receptor 3A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >4PIR_I|Chain F, G, H, I, J|VHH15|Lama glama (9844) DVQLVESGGGLVQPGGSLRLSCAYSGSLFSILRMDWYRQAPGKERELVAGITRDAAGYADSTNYADSVKGRFTISRDSAKNTVYLQMNSLKPEDTAVYYCNADARTITGRADYWGQGTQVTVSS |
Antigen
Chain: C
Mutation: NULL
| >4PIR_C|Chain A, B, C, D, E|5-hydroxytryptamine receptor 3A|Mus musculus (10090) ATQARDTTQPALLRLSDHLLANYKKGVRPVRDWRKPTTVSIDVIMYAILNVDEKNQVLTTYIWYRQYWTDEFLQWTPEDFDNVTKLSIPTDSIWVPDILINEFVDVGKSPNIPYVYVHHRGEVQNYKPLQLVTACSLDIYNFPFDVQNCSLTFTSWLHTIQDINITLWRSPEEVRSDKSIFINQGEWELLEVFPQFKEFSIDISNSYAEMKFYVIIRRRPLFYAVSLLLPSIFLMVVDIVGFCLPPDSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLAETIFIVRLVHKQDLQRPVPDWLRHLVLDRIAWILCLGEQPMAHRPPATFQANKTDDCSAMGNHCSHVGGPQDLEKTPRGRGSPLPPPREASLAVRGLLQELSSIRHFLEKRDEMREVARDWLRVGYVLDRLLFRIYLLAVLAYSITLVTLWSIWHSS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: ASP1 GLN3 GLY26 SER27 LEU28 PHE29 SER30 ARG105 THR106 ILE107 GLY109 ARG110 C: THR38 SER40 TYR67 TRP68 THR69 THR84 HIS118 HIS119 ARG120 GLU122 GLN124 TRP168 ARG169 GLU173 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
