Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 702 |
| PDBID: | 4C59 |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1042/BJ20131399 |
| Antibody | |
| Antibody: | NbGAK_4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cyclin-G-associated kinase |
| Antigen mutation: | No |
| Durg Target: | O14976 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4C59_B|Chain B|NANOBODY|LAMA GLAMA (9844) QVQLQESGGGSVQAGGSLRLSCGASEYTSRMGWFRQAPGAEREGVACIHRQSNLSYYSDSVRGRFTISQDNAKTTAFLLMSSLKPEDTAIYYCATTTDCAAFVERATAITAGQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4C59_A|Chain A|Cyclin-G-associated kinase|Homo sapiens (9606) SMPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: TYR28 SER33 ARG34 HIS57 GLN59 SER60 LEU62 SER63 TYR66 TYR67 ARG72 THR108 ASP109 CYS110 GLU111 ARG112 THR114 A: ARG164 ASN237 LYS243 SER290 LEU291 ALA294 GLN297 GLU301 GLU302 ARG303 LEU304 SER305 ALA307 GLU308 HIS311 GLN312 GLU315 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
