Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7013 |
| PDBID: | 4P2C |
| Chains: | K_F |
| Organism: | Escherichia coli, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.82 |
| Reference: | 10.1074/jbc.M114.566257 |
| Antibody | |
| Antibody: | Stx2e1 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Shiga toxin Stx2e subunit B |
| Antigen mutation: | No |
| Durg Target: | Q47644 |
Sequence information
Antibody
Chain: K
Mutation: NULL
| >4P2C_K|Chain G, H, I, J, K|Nanobody 1, Anti-F4+ETEC bacteria VHH variable region|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCAVSGSIFRLSTMGWYRQAPGKQREFVASITSYGDTNYRDSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNANIEAGTYYGPGRDYWGQGTQVTVSSHHHHHH |
Antigen
Chain: F
Mutation: NULL
| >4P2C_F|Chain B, C, D, E, F|Shiga toxin 2e, subunit B|Escherichia coli (562) ADCAKGKIEFSKYNEDNTFTVKVSGREYWTNRWNLQPLLQSAQLTGMTVTIISNTCSSGSGFAQVKFN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| K: GLY26 SER27 ARG30 LEU31 ILE51 SER53 TYR54 GLY55 ASP56 ARG71 ASN73 GLU100 ALA101 GLY102 THR103 TYR104 F: ASN14 ASP16 ASN17 THR18 THR20 TRP29 ASN31 ARG32 TRP33 ASN34 SER53 ASN54 THR55 GLY59 SER60 GLY61 PHE62 ALA63 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
