Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7005 |
| PDBID: | 4OGX |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.40 |
| Reference: | 10.1074/jbc.M114.569061 |
| Antibody | |
| Antibody: | DX-2930 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Lanadelumab(Approved) |
| Antigen | |
| Antigen: | Plasma kallikrein |
| Antigen mutation: | Yes |
| Durg Target: | P03952 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4OGX_H|Chain B[auth H]|DX-2930 HEAVY CHAIN|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSHYIMMWVRQAPGKGLEWVSGIYSSGGITVYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAYRRIGVPRRDEFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: L
Mutation: NULL
| >4OGX_L|Chain C[auth L]|DX-2930 LIGHT CHAIN|Homo sapiens (9606) DIQMTQSPSTLSASVGDRVTITCRASQSISSWLAWYQQKPGKAPKLLIYKASTLESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQQYNTYWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: N6E/N63E/N104E/C113S
| >4OGX_A|Chain A|Plasma kallikrein|Homo sapiens (9606) IVGGTESSWGEWPWQVSLQVKLTAQRHLCGGSLIGHQWVLTAAHCFDGLPLQDVWRIYSGILELSDITKDTPFSQIKEIIIHQNYKVSEGNHDIALIKLQAPLEYTEFQKPISLPSKGDTSTIYTNCWVTGWGFSKEKGEIQNILQKVNIPLVTNEECQKRYQDYKITQRMVCAGYKEGGKDACKGDSGGPLVCKHNGMWRLVGITSWGEGCARREQPGVYTKVAEYMDWILEKTQSSDGK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 PHE27 THR28 PHE29 SER30 HIS31 ARG100 ILE101 GLY102 VAL103 PRO104 ARG105 ARG106 ASP107 GLU108 ASP110 L: SER31 TRP32 TYR49 LYS50 THR53 LEU54 GLU55 SER56 GLY57 VAL58 A: VAL410 LEU412 ALA414 ARG416 LEU418 CYS419 HIS434 CYS435 ASP437 GLY438 LEU439 VAL477 SER478 GLU479 GLY480 ASP483 PHE524 GLU527 LYS528 ASP554 TYR555 ASP572 ALA573 CYS574 LYS575 GLY576 SER578 THR596 SER597 TRP598 GLY599 GLU600 GLY601 CYS602 ARG604 GLN607 GLY609 TYR611 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
