Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 7002 |
| PDBID: | 4OCN |
| Chains: | C_B |
| Organism: | Saccharomyces cerevisiae S288C, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.25 |
| Reference: | 10.1073/pnas.1400546111 |
| Antibody | |
| Antibody: | 1 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | 26S proteasome regulatory subunit RPN11 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >4OCN_C|Chain C, F|Nb1|Lama glama (9844) MQVQLQESGGGLVPAGGSLRLSCVDSGRTFSSTVMAWFRQAPGKEREFVATIRWSGGNTYYADSVKGRFTISRDNARNTVYLQMNSLKPEDTAVYYCAGGTYYGTLSYKYDFWGRGTQVTVSSHHHHHHEPEA |
Antigen
Chain: B
Mutation: NULL
| >4OCN_B|Chain B, E|26S proteasome regulatory subunit RPN11|Saccharomyces cerevisiae (559292) MERLQRLMMNSKVGSADTGRDDTKETVYISSIALLKMLKHGRAGVPMEVMGLMLGEFVDDYTVNVVDVFAMPQSGTGVSVEAVDDVFQAKMMDMLKQTGRDQMVVGWYHSHPGFGCWLSSVDVNTQKSFEQLNSRAVAVVVDPIQSVKGKVVIDAFRLIDTGALINNLEPRQTTSNTGLLNKANIQALIHGLNRHYYSLNIDYHKTAKETKMLMNLHKEQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ARG53 ASN58 THR59 TYR60 THR101 TYR102 TYR103 GLY104 THR105 LEU106 TYR108 LYS109 TYR110 ASP111 B: TYR28 SER30 GLU56 PHE57 VAL58 ASP59 TYR61 THR62 ASN64 VAL66 GLY99 ARG100 GLN102 HIS204 LYS205 THR206 ALA207 LYS208 GLU209 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
