Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 699 |
| PDBID: | 4C57 |
| Chains: | C_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.1042/BJ20131399 |
| Antibody | |
| Antibody: | NbGAK_4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cyclin-G-associated kinase |
| Antigen mutation: | No |
| Durg Target: | O14976 |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >4C57_C|Chain C, D|NANOBODY|LAMA GLAMA (9844) QVQLQESGGGLVQPGGSLRLSCSASGFKFNDSYMSWVRRVPGKGLEWVAGIWEDSSAAHYRDSVKGRFTISRDNAKNMLYLQMSSLKSDDTGLYYCVRRGYSGDYRPINNPSSQGTQVTVSSAAAYPYDVPDYGSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4C57_A|Chain A, B|Cyclin-G-associated kinase|Homo sapiens (9606) SMPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGPPPPV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP32 TYR34 TRP57 GLU58 ALA62 HIS66 ARG107 GLY108 TYR109 SER110 TYR111 ARG112 PRO113 ILE114 ASN116 A: ARG41 ARG43 ARG44 LEU46 GLU56 GLN58 ASP59 VAL60 GLY61 SER62 GLY63 ARG64 GLU65 GLU124 LEU125 LYS127 ASN183 GLN184 LEU332 GLU333 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
