Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6967 |
| PDBID: | 4MQT |
| Chains: | B_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/nature12735 |
| Antibody | |
| Antibody: | 9-8 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | M2 muscarinic acetylcholine receptor |
| Antigen mutation: | Yes |
| Durg Target: | P08172 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >4MQT_B|Chain B|Nanobody 9-8|Lama glama (9844) GPGSQVQLQESGGGLVQAGDSLRLSCAASGFDFDNFDDYAIGWFRQAPGQEREGVSCIDPSDGSTIYADSAKGRFTISSDNAENTVYLQMNSLKPEDTAVYVCSAWTLFHSDEYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: N17D/N18D/N21D/N24D/P250T/S251R
| >4MQT_A|Chain A|Muscarinic acetylcholine receptor M2|Homo sapiens (9606) DYKDDDENLYFQGLEMDDSTDSSDNSLALTSPYLEKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHISRASKSRIKKDKKEPVANQDPVSTRKKPPPSREKKVTRTILAILLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYINSTINPACYALCNATFKKTFKHLLMCHYKNIGATRHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN1 VAL2 PHE29 ASP30 ASP33 ASP34 PHE40 GLU47 ARG48 GLU49 GLY50 SER52 CYS53 ASP55 SER57 ASP58 ILE62 TYR63 ALA64 TRP102 THR103 LEU104 PHE105 HIS106 SER107 ASP108 GLU109 TYR110 TRP111 A: HIS53 LEU54 THR56 ASN58 ASN59 ARG121 CYS124 VAL125 PRO128 LEU129 PRO132 VAL133 ARG135 TYR206 ILE209 SER380 ARG381 LYS384 VAL385 THR388 ILE389 CYS443 ASN444 ALA445 THR446 PHE447 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
