Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6961 |
| PDBID: | 4MHJ |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/goose/Guangdong/1/1996(H5N1)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 6.98 |
| Reference: | 10.1128/JVI.01577-13 |
| Antibody | |
| Antibody: | H5M9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 hemagglutinin HA1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4MHJ_H|Chain S[auth F], T[auth H], U[auth K], V[auth R], W[auth T], X[auth W]|H5M9 antibody, heavy chain (IgG1)|Mus musculus (10090) EVHLQQSGPELVKPGASVKMSCKTSGYTFTEYTIHWMKQSHGKSLEWIGGIFPNNGDTTYNQKFKVRATLTVGRSSSTAYMDLRSLTSEDSAVYYCVRNYGSSYGYFDVWGAGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >4MHJ_L|Chain K[auth E], L[auth J], M[auth L], N[auth Q], O[auth V], P[auth X]|H5M9 antibody, light chain (kappa)|Mus musculus (10090) DIVLTQSPGSLTVSLGQRATISCRASESVDNFGKSFMHWYQQKPGQSPKLLIYRASNREFGIPARFNGSGSGTDFALTINPVEADDVATYFCQQSNEDPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4MHJ_A|Chain A, C, E[auth G], F[auth M], H[auth O], J[auth S]|Hemagglutinin HA1 chain|Influenza A virus (93838) ADPGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKTHNGKLCDLNGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKASPANDLCYPGDFNDYEELKHLLSRTNHFEKIQIIPKSSWSNHDASSGVSSACPYHGRSSFFRNVVWLIKKNSAYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPEIATRPKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSAIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNTPQRERRRKKR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PHE28 LYS30 PHE32 TYR49 ARG50 ASN53 ARG54 GLU55 GLY57 ILE58 H: GLU1 VAL2 GLY26 TYR27 THR28 THR30 GLU31 TYR32 THR33 ARG94 TYR96 GLY97 SER99 ASP101 A: LYS50 ASP53 GLY55 VAL56 PRO58 LEU59 ILE60 ARG62 ASP77 GLU78 ILE80 ASN81 GLU83 HIS117 GLU119 TYR141 HIS142 LYS259 LYS261 GLU273 TYR274 GLY275 ASN276 CYS277 ASN278 LYS280 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
