Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6957 |
| PDBID: | 4MHH |
| Chains: | HL_E |
| Organism: | Influenza A virus (A/Viet Nam/1203/2004(H5N1)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.56 |
| Reference: | 10.1128/JVI.01577-13 |
| Antibody | |
| Antibody: | H5M9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H5N1 hemagglutinin HA1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4MHH_H|Chain J[auth G], K[auth H], L[auth J]|H5M9 antibody, heavy chain (IgG1)|Mus musculus (10090) EVHLQQSGPELVKPGASVKMSCKTSGYTFTEYTIHWMKQSHGKSLEWIGGIFPNNGDTTYNQKFKVRATLTVGRSSSTAYMDLRSLTSEDSAVYYCVRNYGSSYGYFDVWGAGTTVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >4MHH_L|Chain D[auth I], E[auth K], F[auth L]|H5M9 antibody, light chain (kappa)|Mus musculus (10090) DIVLTQSPGSLTVSLGQRATISCRASESVDNFGKSFMHWYQQKPGQSPKLLIYRASNREFGIPARFNGSGSGTDFALTINPVEADDVATYFCQQSNEDPRTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >4MHH_E|Chain A, B[auth C], C[auth E]|Hemagglutinin HA1 chain|Influenza A virus (284218) ADPGDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKKHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRERRRKKR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PHE28 LYS30 PHE32 TYR49 ARG50 ASN53 ARG54 GLU55 PHE56 H: GLY26 TYR27 THR28 THR30 GLU31 TYR32 THR33 ASN53 ASN54 ASP56 TYR96 GLY97 SER98 SER99 E: LYS50 ASP53 GLY55 VAL56 LYS57 PRO58 LEU59 ILE60 ARG62 GLU78 PHE79 ASN81 VAL82 GLU83 TRP84 HIS117 GLU119 LYS120 LYS261 GLU273 TYR274 GLY275 ASN276 CYS277 ASN278 LYS280 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
