Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6953 |
| PDBID: | 4M1G |
| Chains: | HL_B |
| Organism: | Mus musculus, Vaccinia virus |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.1371/journal.ppat.1005148 |
| Antibody | |
| Antibody: | A27D7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vaccinia virus A33 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4M1G_H|Chain B[auth H]|Murine IgG2a A27D7 Heavy chain Fab domain|Mus musculus (10090) QVQLKESGPGLVAPSQSLSITCTVSGFSLTDYGVSWIRQPPGKGLEWLGVTWGGGTTYYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTAMYYCAKHKASYNGLDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGP |
Light Chain: L
Mutation: NULL
| >4M1G_L|Chain A[auth L]|Murine IgG2a A27D7 Light chain Fab domain|Mus musculus (10090) ELVLTQSPTTMAASPGEKITITCSASSSISSNYLHWYQQKPGFSPKLLIYRTSNLASGVPARFSGSGSGTSYSLTIGTMEAEDVATYYCQQGSSIPFTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: B
Mutation: S1M/L30M/K35A/L52M
| >4M1G_B|Chain C[auth A], D[auth B]|A33R|Vaccinia virus (10245) MSTTQYDHKESCNGLYYQGSCYILHSDYQMFSDAAANCTAESSTLPNKSDVMITWLIDYVEDTWGSDGNPITKTTSDYQDSDVSQEVRKYFCVKTMN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: LEU2 SER28 SER30 ASN32 TYR33 GLY92 SER93 SER94 ILE95 PRO96 PHE97 H: TRP52 GLY53 GLY54 THR56 TYR58 ALA100 SER101 TYR102 ASN103 B: SER114 ASP115 TYR116 GLN117 MET118 ASP121 ASP150 GLN173 GLU174 VAL175 ARG176 LYS177 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
