Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 695 |
| PDBID: | 3WSQ |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.50 |
| Reference: | 10.4161/mabs.28786 |
| Antibody | |
| Antibody: | F0178C1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Receptor tyrosine-protein kinase erbB-2 |
| Antigen mutation: | No |
| Durg Target: | P04626 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3WSQ_H|Chain C[auth H]|Antibody Heavy Chain|Homo sapiens (9606) EVQLVQSGGGLKVPGGSVKVSCKASSYTFTSYGISWVRQAPGQGLEWMGDVNTYAGNTNYAQKLQGRVTMTTDTGTSTAYMELRSLRSDDAAVYYCARDRGYYAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >3WSQ_L|Chain B[auth L]|Antibody Light Chain|Homo sapiens (9606) QSVLTQPPSASGTPGQTVTLSCSGSRTNAGRDPVSWYQQLPGTAPKLLTSTAPQGPSGVPDRFSGSKSGTSSSLAISGLQSGDEADYYCTVWDSVPGASVFGSGTFLTVTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >3WSQ_A|Chain A|Receptor tyrosine-protein kinase erbB-2|Homo sapiens (9606) TQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLY30 ARG31 ASP32 GLN54 TRP92 ASP93 SER94 VAL95 H: THR28 THR30 SER31 TYR32 ASP50 ASN52 TYR54 THR58 ASN59 THR74 ASP99 ARG100 GLY101 TYR102 TYR103 A: ARG12 LEU13 ALA15 GLU330 ARG332 LEU355 PRO356 GLU357 PHE359 ASP360 GLY361 ASP362 PRO363 ALA364 ASN366 PRO369 GLN371 ALA392 ASP395 GLN424 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
