Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6943 |
| PDBID: | 4LQF |
| Chains: | HL_A |
| Organism: | Vaccinia virus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.30 |
| Reference: | 10.1371/journal.ppat.1005148 |
| Antibody | |
| Antibody: | A2C7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Vaccinia virus A33 |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4LQF_H|Chain B[auth H]|Murine IgG2b A2C7 Heavy chain Fab domain|Mus musculus (10090) EVKLVESGGGLVQPGGSLTLSCATSGFTFSDYYIYWVRQTPEKRLEWVAYISNSGGNTYYSDAVKGRFTISRDNAKNTLFLQMSRLKSEDTAVYYCARQWGGAMDYWGQGTSVTVSSAKTTPPSVYPLAPGCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEP |
Light Chain: L
Mutation: NULL
| >4LQF_L|Chain C[auth L]|Murine IgG2b A2C7 Light chain Fab domain|Mus musculus (10090) DVVMTQTPLSLPVSLGDQASISCRSSQSLIHTNGNTYLHWYLQKPGQSPKLLIYKVSNRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYFCSQSTHIPPWTFGGGTKLEIKRADAAPTVSIFPTISEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: A
Mutation: S1M/L30M/K35A/L52M
| >4LQF_A|Chain A|A33R|Vaccinia virus (10245) MSTTQYDHKESCNGLYYQGSCYILHSDYQMFSDAAANCTAESSTLPNKSDVMITWLIDYVEDTWGSDGNPITKTTSDYQDSDVSQEVRKYFCVKTMN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASP31 TYR33 TYR35 TYR50 SER52 ASN57 TYR59 GLN99 TRP100 GLY101 GLY102 L: HIS31 THR32 ASN33 TYR37 LYS55 SER96 THR97 HIS98 ILE99 PRO100 TRP102 A: TYR116 GLN117 MET118 PHE119 SER120 ASP121 SER154 ASP155 GLY156 ASN157 SER169 ASP170 SER172 GLN173 GLU174 VAL175 ARG176 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
