Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6940 |
| PDBID: | 4LIQ |
| Chains: | HL_E |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1016/j.ccr.2014.05.016 |
| Antibody | |
| Antibody: | RG7155 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Emactuzumab(Phase-III) |
| Antigen | |
| Antigen: | Macrophage colony-stimulating factor 1 receptor (CSF-1R) |
| Antigen mutation: | No |
| Durg Target: | P07333 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4LIQ_H|Chain B[auth H]|Fab fragment RG7155 heavy chain|Mus musculus (10090) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYDISWVRQAPGQGLEWMGVIWTDGGTNYAQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCARDQRLYFDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: L
Mutation: NULL
| >4LIQ_L|Chain C[auth L]|Fab fragment RG7155 light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASEDVNTYVSWYQQKPGKAPKLLIYAASNRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSFSYPTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >4LIQ_E|Chain A[auth E]|Macrophage colony-stimulating factor 1 receptor|Homo sapiens (9606) MGSGPGVLLLLLVATAWHGQGIPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDEAAALEVLFQGPGTHHHHHHHHHHIGLNDIFEAQKIEWHE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: SER17 ASP33 TRP47 VAL50 TRP52 THR53 ASP54 GLY56 THR57 ASN58 GLN61 GLN64 GLY65 THR68 GLU81 ARG83 SER84 ASP98 ARG100 LEU101 L: ASP28 ASN30 TYR32 SER91 PHE92 SER93 TYR94 PRO95 E: THR314 VAL315 GLY316 GLU317 GLY318 LEU319 ASN320 LYS350 ALA352 SER367 PRO369 ARG370 LEU371 LYS372 PRO373 GLU375 TYR400 PRO401 PRO402 GLU403 ASP439 ARG440 VAL491 GLY492 SER493 GLY494 SER495 TRP496 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
