Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 694 |
| PDBID: | 3WIH |
| Chains: | MI_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | 10.1002/pro.2619 |
| Antibody | |
| Antibody: | B2212A Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Roundabout homolog 1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >3WIH_M|Chain B[auth L], E[auth M]|anti-human ROBO1 antibody B2212A Fab light chain|Mus musculus (10090) DIQMTQTTSSLSASLGDRVTISCRASQDISNFLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDFSLTISKLEQEDIATYFCQQGNTLPLTFGAGTKLELKRAEAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Light Chain: I
Mutation: NULL
| >3WIH_I|Chain C[auth H], F[auth I]|anti-human ROBO1 antibody B2212A Fab heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKISCKASGYTFTDYYMNWVKLSHGKSLEWIGDIVPNNGDTTYNQNFRGKATLTVDKSSSTAYMELRSLTSEDSAVYYCARFSNYVYPFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAILQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPR |
Antigen
Chain: B
Mutation: NULL
| >3WIH_B|Chain A, D[auth B]|Roundabout homolog 1|Homo sapiens (9606) APPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| M: PHE32 LEU46 TYR49 TYR50 ARG53 LEU54 HIS55 SER60 ASN92 I: TYR27 THR28 THR30 ASP31 TYR32 SER100 ASN101 TYR102 VAL103 TYR104 PRO105 ASP107 B: ASN17 GLY18 THR19 ALA20 GLU39 THR49 ARG50 TYR51 HIS52 ILE53 ASN54 LYS55 THR56 PRO67 PHE68 LEU69 VAL70 PRO71 TYR75 ASP99 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
