Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6938 |
| PDBID: | 4LF3 |
| Chains: | BA_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.73 |
| Reference: | 10.1074/jbc.M113.496984 |
| Antibody | |
| Antibody: | mAb1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glucagon receptor (GCGR) |
| Antigen mutation: | Yes |
| Durg Target: | P47871 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >4LF3_B|Chain B, E|Fab light chain|Mus musculus (10090) QVQLVESGGGVVQPGRSLRLSCAASGFTFSSYGMHWVRQAPGKGLEWVAVMWYDGSNKDYVDSVKGRFTISRDNSKNTLYLQMNRLRAEDTAVYYCAREKDHYDILTGYNYYYGLDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: A
Mutation: NULL
| >4LF3_A|Chain A, D|Fab heavy chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCRASQGIRNDLGWYQQKPGKAPKRLIYAASSLESGVPSRFSGSGSGTEFTLTISSVQPEDFVTYYCLQHNSNPLTFGGGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: G12S
| >4LF3_C|Chain C, F|Glucagon receptor|Homo sapiens (9606) MDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: HIS91 ASN92 SER93 ASN94 LEU96 B: HIS249 VAL264 TRP266 ASP268 SER270 ASN271 GLU313 TYR317 ILE319 LEU320 THR321 GLY322 TYR323 ASN324 TYR326 C: LEU32 PHE33 TRP36 LYS64 TYR65 TYR84 LEU85 PRO86 TRP87 ARG108 PRO110 ARG111 GLY112 GLN113 PRO114 TRP115 ARG116 ALA118 GLN122 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
