Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6937 |
| PDBID: | 4LEO |
| Chains: | AB_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.64 |
| Reference: | 10.1158/0008-5472.CAN-13-0099 |
| Antibody | |
| Antibody: | RG7116 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Lumretuzumab(Phase-I/II) |
| Antigen | |
| Antigen: | The EGF receptor (EGFR) HER3 ECD |
| Antigen mutation: | No |
| Durg Target: | P21860 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4LEO_A|Chain A|RG7116 Fab heavy chain|Mus musculus (10090) QVQLVQSGAEVKKPGASVKVSCKASGYTFRSSYISWVRQAPGQGLEWMGWIYAGTGSPSYNQKLQGRVTMTTDTSTSTAYMELRSLRSDDTAVYYCARHRDYYSNSLTYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTH |
Light Chain: B
Mutation: NULL
| >4LEO_B|Chain B|RG7116 Fab light chain|Mus musculus (10090) DIVMTQSPDSLAVSLGERATINCKSSQSVLNSGNQKNYLTWYQQKPGQPPKLLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQSDYSYPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >4LEO_C|Chain C|Receptor tyrosine-protein kinase erbB-3|Homo sapiens (9606) SEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGAAALEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: TYR33 TRP50 TYR52 THR55 GLY56 SER57 PRO58 SER59 TYR60 GLN62 THR69 TYR102 TYR103 ASN105 B: ASN31 SER32 GLY33 ASN34 LYS36 TYR38 ASP97 TYR98 SER99 TYR100 TYR102 C: LEU17 LEU48 MET72 TYR92 ASP93 LYS95 PHE96 PHE99 MET101 LEU102 TYR104 THR106 ASN107 TYR129 GLU131 LYS132 ASP134 ARG145 GLU154 ILE155 VAL156 VAL157 LYS158 ASP159 ARG162 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
