Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6910 |
| PDBID: | 4KI5 |
| Chains: | EF_M |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.47 |
| Reference: | 10.1182/blood-2013-08-519124 |
| Antibody | |
| Antibody: | G99 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | The factor VIII C2 domain |
| Antigen mutation: | No |
| Durg Target: | P00451; P00451; |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >4KI5_E|Chain C[auth E]|MURINE MONOCLONAL G99 FAB HEAVY CHAIN|Mus musculus (10090) QVQLQQSGAELMKPGASVKISCKATGYTFSSYWIEWVKQRPGHGLEWIGEILPGSGSTNYNERFKGKASFTADSSSNTAYMQLSSLTSEDSAVYYCTRTSYYFGSSYDFDVWGAGTTVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPALLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGP |
Light Chain: F
Mutation: NULL
| >4KI5_F|Chain D[auth F]|MURINE MONOCLONAL G99 FAB LIGHT CHAIN|Mus musculus (10090) DIQMTQSPSSLSASLGERVSLTCRASQEISGYLSWLQQKPDGTIKRLIYAASTLDSSVPKRFSGSRSGSDYSLTISSLDSEDFAVYYCLQYASYPYTFGGGTKVEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: M
Mutation: NULL
| >4KI5_M|Chain E[auth M]|Coagulation factor VIII|Homo sapiens (9606) MGSSHHHHHHSSGLVPRGSHMLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRSNAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQDGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQIALRMEVLGCEAQDLY |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: SER30 SER31 TRP33 LEU52 PRO53 GLY54 SER55 GLY56 SER57 ASN59 THR99 SER100 TYR101 TYR102 PHE103 GLY104 F: GLU28 SER30 TYR32 TYR91 ALA92 SER93 TYR94 TYR96 M: SER2193 SER2194 GLN2222 VAL2223 ASN2224 ASN2225 PRO2226 LYS2227 GLU2228 TRP2229 LEU2261 SER2263 HIS2269 TRP2271 LEU2273 PHE2275 LYS2279 VAL2280 VAL2282 ARG2307 HIS2309 GLN2311 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
