Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 691 |
| PDBID: | 3W2D |
| Chains: | HL_A |
| Organism: | Staphylococcus aureus, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.4161/mabs.27106 |
| Antibody | |
| Antibody: | 3E2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Staphylococcal Eenterotoxin B |
| Antigen mutation: | No |
| Durg Target: | P01552 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3W2D_H|Chain C[auth H]|Monoclonal Antibody 3E2 Fab figment heavy chain|Mus musculus (10090) DVQLQESGPGLVRPSQSLSLTCTVTGYSLTSDFAWNWIRQFPGNKLEWMGYINYSGYTNYNPSLKSRISITRDTSKNQFFLQLNSVTTEDTATYYCAREAYYGRAYWFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >3W2D_L|Chain B[auth L]|Monoclonal Antibody 3E2 Fab figment light chain|Mus musculus (10090) ENVLTQSPAIMSASPGETVTMTCRATSSVSSTYLHWYQQKSGASPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISSVEAEDAATYYCQQYINYPLTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >3W2D_A|Chain A|Enterotoxin type B|Staphylococcus aureus (1280) ESQPDPKPDELHKSSKFTGLMENMKVLYDDNHVSAINVKSIDQFLYFDLIYSIKDTKLGNYDNVRVEFKNKDLADKYKDKYVDVFGANYYYQCYFSKKTNDINSHQTDKRKTCMYGGVTEHNGNQLDKYRSITVRVFEDGKNLLSFDVQTNKKKVTAQELDYLTRHYLVKNKKLYEFNNSPYETGYIKFIENENSFWYDMMPAPGDKFDQSKYLMMYNDNKMVDSKDVKIEVYLTTKKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: GLU23 TYR55 TYR114 ILE115 ASN116 TYR117 LEU119 H: ALA52 TRP66 TYR69 ASN71 TYR72 TYR75 THR76 ASN77 PRO80 LYS83 GLU117 GLY121 ARG122 TYR124 A: GLN70 PHE71 LEU72 TYR73 PHE74 GLU94 PHE95 LYS96 ASN97 LYS98 ASP99 ASP102 LYS103 LYS105 LYS107 LYS125 MET242 ASN245 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
