Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6900 |
| PDBID: | 4K3J |
| Chains: | HL_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1073/pnas.1302725110 |
| Antibody | |
| Antibody: | Onartuzumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Onartuzumab(Phase-III) |
| Antigen | |
| Antigen: | Hepatocyte growth factor (HGF) beta chain |
| Antigen mutation: | No |
| Durg Target: | P08581 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4K3J_H|Chain C[auth H]|Onartuzumab Fab heavy chain|Mus musculus (10090) EVQLVESGGGLVQPGGSLRLSCAASGYTFTSYWLHWVRQAPGKGLEWVGMIDPSNSDTRFNPNFKDRFTISADTSKNTAYLQMNSLRAEDTAVYYCATYRSYVTPLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >4K3J_L|Chain D[auth L]|Onartuzumab Fab light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCKSSQSLLYTSSQKNYLAWYQQKPGKAPKLLIYWASTRESGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYAYPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >4K3J_B|Chain B|Hepatocyte growth factor beta chain|Homo sapiens (9606) MKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAHHHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TRP33 HIS35 MET50 SER53 ASN54 ASP56 THR57 ARG58 PHE59 ASP65 TYR95 TYR98 L: ILE2 SER27 SER28 TYR32 TRP50 TYR91 TYR92 ALA93 TYR94 TRP96 B: PRO325 GLY326 ALA327 GLN328 ARG331 GLN332 ILE333 GLY334 ALA335 SER336 LEU337 ASN338 ASP339 LYS368 TYR369 ASP372 LYS376 ARG426 ILE446 GLY448 ASP449 ARG469 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
