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Entry information

Complex
AACDB_ID: 690
PDBID: 3W11
Chains: CD_E
Organism: Homo sapiens, Mus musculus
Method: XRD
Resolution (Å): 3.90
Reference: 10.1038/nature11781
Antibody
Antibody: 83-7 Fab
Antibody mutation: No
INN (Clinical Trial):
Antigen
Antigen: Insulin receptor domains L1-CR
Antigen mutation: No
Durg Target: P06213

Sequence information

Antibody

Heavy Chain: C
Mutation: NULL

>3W11_C|Chain C|monoclonal antibody fab 83-7 fragment - heavy chain|Mus musculus (10090)
QVQLKESGPGLVAPSQSLSITCTVSGFPLTAYGVNWVRQPPGKGLEWLGMIWGDGNTDYNSALKSRLSISKDNSKSQVFLKMNSLQTDDTARYYCARDPYGSKPMDYWGQGTSVTVSS

Light Chain: D
Mutation: NULL

>3W11_D|Chain D|monoclonal antibody fab 83-7 fragment - light chain|Mus musculus (10090)
DIVMSQSPSSLVVSVGEKVTMSCKSSQSLLYSSNQKNFLAWYQQKPGQSPKLLIYWASTRESGVPDRFTGSGSGTDFTLTISSVKAEDLAVYYCQQYFRYRTFGGGTKLEIKRA

Antigen

Chain: E
Mutation: NULL

>3W11_E|Chain E|Insulin receptor domains L1-CR|Homo sapiens (9606)
HLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNHIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPK

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.

Interacting residues (ΔSASA based)

C: PRO28 THR30 ALA31 TYR32 TRP52 GLY53 ASP54 ASN56 PRO99 TYR100 GLY101 SER102

D: TYR31 SER32 SER33 ASN34 GLN35 LYS36 PHE38 TRP56 TYR97 PHE98 ARG99

E: GLU211 ASP221 PRO222 THR223 LYS224 LEU233 ASP234 GLY235 ARG236 CYS237 VAL238 GLU239 THR240 CYS241 PRO242 PRO243 PRO244 TYR245 HIS247 ASN281 ASN282 LYS283

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

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