Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6899 |
| PDBID: | 4K24 |
| Chains: | HL_U |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 4.50 |
| Reference: | 10.1371/journal.pone.0085349 |
| Antibody | |
| Antibody: | ATN-658 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Urokinase plasminogen activator surface receptor (uPAR) |
| Antigen mutation: | No |
| Durg Target: | Q03405 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4K24_H|Chain C[auth H]|anti-uPAR antibody, heavy chain|Mus musculus (10090) EVQLQQSGPELVKTGASVKISCKASGYSFTSYYMHWVKQSHGKSLEWIGEINPYNGGASYNQKIKGRATFTVDTSSRTAYMQFNSLTSEDSAVYYCARSIYGHSVLDYWGQGTSVSVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCGCKPCIC |
Light Chain: L
Mutation: NULL
| >4K24_L|Chain D[auth L]|anti-uPAR antibody, light chain|Mus musculus (10090) DVVMTQTPLTLSVTIGQPASISCKSSQSLLDSDGKTYLNWLLQRPGQSPKRLIYLVSKLDSGVPDRFTGSGSGTDFTLKISRVEAEDLGVYYCWQGTHFPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: U
Mutation: NULL
| >4K24_U|Chain E[auth U]|Urokinase plasminogen activator surface receptor|Homo sapiens (9606) RSLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY26 TYR27 SER28 THR30 SER31 TYR32 TYR33 TYR53 ASN54 ARG94 ILE96 TYR97 GLY98 HIS99 L: SER27 ASP28 LYS30 ARG46 TYR49 LEU50 U: GLN193 TYR195 SER207 GLU208 THR210 PHE211 LEU212 ILE213 ASP214 CYS266 THR267 LYS268 SER269 GLY270 CYS271 HIS273 ASP275 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
