Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6898 |
| PDBID: | 4JZJ |
| Chains: | AB_D |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1016/j.celrep.2014.06.038 |
| Antibody | |
| Antibody: | CSL362 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Talacotuzumab(Phase-II) |
| Antigen | |
| Antigen: | Interleukin-3 receptor subunit alpha |
| Antigen mutation: | No |
| Durg Target: | P26951 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4JZJ_A|Chain C[auth A], E[auth H]|Fab Heavy Chain|Mus musculus (10090) EVQLVQSGAEVKKPGESLKISCKGSGYSFTDYYMKWARQMPGKGLEWMGDIIPSNGATFYNQKFKGQVTISADKSISTTYLQWSSLKASDTAMYYCARSHLLRASWFAYWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: B
Mutation: NULL
| >4JZJ_B|Chain D[auth B], F[auth L]|Fab Light Chain|Mus musculus (10090) DIVMTQSPDSLAVSLGERATINCESSQSLLNSGNQKNYLTWYQQKPGQPPKPLIYWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQNDYSYPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >4JZJ_D|Chain A[auth C], B[auth D]|Interleukin-3 receptor subunit alpha|Homo sapiens (9606) KEDPNPPITNLRMKAKAQQLTWDLNRNVTDIECVKDADYSMPAVNNSYCQFGAISLCEVTNYTVRVANPPFSTWILFPENSGKPWAGAENLTCWIHDVDFLSCSWAVGPGAPADVQYDLYLNVAKRQQYECLHYKTDAQGTRIGCRFDDISRLSSGSQSSHILVRGRSAAFGIPCTDKFVVFSQIEILTPPNMTAKCNKTHSFMHWKMRSHFNRKFRYELQIQKRMQPVITEQVRDRTSFQLLNPGTYTVQIRARERVYEFLSAWSTPQRFECDQEEGVNTRAWRTS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR30 ASP31 TYR32 TYR33 TRP47 ASP50 ILE52 PRO53 SER54 ASN55 ALA57 PHE59 TYR60 GLN62 HIS100 LEU101 LEU102 ARG103 ALA104 SER105 B: ASP1 ASN31 GLY33 TYR38 TRP56 TYR98 SER99 TYR100 PRO101 TYR102 D: THR48 ASP49 GLU51 VAL53 ALA56 ASP57 TYR58 SER59 MET60 PRO61 ALA62 VAL63 THR82 ARG84 VAL85 ALA86 ASN87 PRO88 PRO89 PHE90 SER91 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
