Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6895 |
| PDBID: | 4JRE |
| Chains: | HL_D |
| Organism: | Escherichia coli K-12, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1038/nature12139 |
| Antibody | |
| Antibody: | 4JR9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Nitrate/nitrite transport protein NarK |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4JRE_H|Chain B, E[auth H]|Immunoglobulin Gamma-2a, Heavy chain|Mus musculus (10090) QIQLVQSGPGLKKPGQTVKISCKASGYSFTDYGMNWVKQAPGKGLEWMGWINTSNGYTTYGAAFKGRFSFSVDNSASTAYLQLSNLKTADTAVYFCARSWYNRAMDYWGQGTSVTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEP |
Light Chain: L
Mutation: NULL
| >4JRE_L|Chain C, F[auth L]|Immunoglobulin Kappa, Light chain|Mus musculus (10090) DILMTQSPSSLSVSVGSSVTITCQASQNITNYIVWYQQKPGQAPKLLIYYTSTLESGIPSRFSGSGSGRDYSFTISNLQPEDVATYYCLQYNSLLTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKNINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: D
Mutation: NULL
| >4JRE_D|Chain A, D|Nitrite extrusion protein 1|Escherichia coli (83333) GSHMSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACVVITWAVYGRHSKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR30 ASP31 TYR32 GLY33 TRP50 ASN52 THR53 SER54 ASN55 TRP100 TYR101 ASN102 ARG103 L: TYR32 TYR49 TYR50 THR53 TYR91 ASN92 D: ALA13 VAL14 ILE15 THR16 ASP17 TRP18 ARG19 ASP22 ALA24 PHE25 GLN27 GLN28 ARG29 ARG32 ALA238 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
