Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6884 |
| PDBID: | 4J4P |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.91 |
| Reference: | 10.1038/nsmb.2795 |
| Antibody | |
| Antibody: | 4J4P Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | IgE-Fc |
| Antigen mutation: | Yes |
| Durg Target: | P01854 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4J4P_H|Chain C, E[auth H]|Immunoglobulin G Fab Fragment Heavy Chain|Homo sapiens (9606) MEWIWIFLFLLSVTTGVHSQVQLQQSGPGLVKPSQTLSLTCGISGDSVSSNSAAWNWLRQSPSRGLEWLGRTYYRSKWYNDYAVSMKSRITINPDTSRNQFSLQLNSVTPEDTAVYYCARDGEISYDYYYYGMDVWGRGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >4J4P_L|Chain D, F[auth L]|Immunoglobulin G Fab Fragment Light Chain|Homo sapiens (9606) MDWSPLLLTLLAHCTGSWAQSVLTQPPSASGTPGQRVTISCSGSSSNIGNNGVNWYQQVPGKPPKLLIYYDDLLPSGVSDRFSGSKSGTSASLAISGLQSEDEADYYCEAWDDSLDGVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: N42Q/N148Q
| >4J4P_A|Chain A, B|Ig epsilon chain C region|Homo sapiens (9606) CSRDFTPPTVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTIQITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVQLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ARG71 TYR73 TYR74 ARG75 SER76 LYS77 TRP78 TYR79 ASP81 ASN93 PRO94 THR96 SER97 ASP121 TYR129 TYR131 MET133 L: ASN50 ASN51 GLY52 ASN54 GLU109 TRP111 ASP113 ASP116 VAL118 A: HIS246 PRO249 GLN301 LYS302 LEU305 SER306 ASP307 LYS327 ASP330 ALA364 PRO365 SER366 LYS367 LYS388 GLU390 LYS391 GLN392 ARG393 ASN394 GLY395 THR396 HIS424 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
