Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6878 |
| PDBID: | 4IDJ |
| Chains: | HL_A |
| Organism: | Staphylococcus aureus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.36 |
| Reference: | 10.1016/j.jmb.2013.02.008 |
| Antibody | |
| Antibody: | LTM14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Alpha-hemolysin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4IDJ_H|Chain B[auth H]|Fab Heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKTSGGTFNNVAINWVRQAPGQGLEWMGGIIPGLDTPNYAQKFQGRVTITADKSTTSTYLELSSLRSDDTAVYYCAREMEVSGRWRPTEAFEIWGQGTMVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSNFGTQTYTCNVDHKPSNTKVDKTVERKAAAHHHHHHHHHH |
Light Chain: L
Mutation: NULL
| >4IDJ_L|Chain C[auth L]|Fab Light chain|Homo sapiens (9606) ETTLTQSPGTLSLSPGERATLSCRASQTISNNFVAWYQQKPGQAPRLLIYGASTRATGIPDRFSGSGSGTDFTLTISSLEPEDFAVYYCQQYGSSPYTFGQGTKVDIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4IDJ_A|Chain A|Alpha-hemolysin|Staphylococcus aureus (1280) GSADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMLKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLY27 THR28 PHE29 ASN30 ASN31 GLY53 LEU54 THR56 PRO57 ASN58 TYR59 GLN61 GLU95 GLU97 L: GLN27 GLY92 SER93 SER94 TYR96 A: LYS30 GLU31 ASN32 GLN64 TYR65 ARG66 VAL67 TYR68 SER69 GLU70 GLU71 GLY72 ALA79 LYS205 ALA207 ASP208 PHE210 LEU211 ASP212 PRO213 ARG253 TRP274 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
