Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6877 |
| PDBID: | 4I9W |
| Chains: | GF_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.75 |
| Reference: | 10.1073/pnas.1218950110 |
| Antibody | |
| Antibody: | 13E9 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | TWIK-related arachidonic acid-stimulated K(+) channel (TRAAK) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: G
Mutation: NULL
| >4I9W_G|Chain D[auth E], F[auth G]|ANTIBODY FAB FRAGMENT HEAVY CHAIN|Mus musculus (10090) EVQLQQSGPELVKPGASMKTSCKVSGYSFTGYIMNWVKQRHGKNLEWIGLINPNTGYTTYNQKFKGKATLTVDKSSSTAYMELLSLTSEDSAIYYCTRGNYVFDYWGQGTTLTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRD |
Light Chain: F
Mutation: NULL
| >4I9W_F|Chain C[auth D], E[auth F]|ANTIBODY FAB FRAGMENT LIGHT CHAIN|Mus musculus (10090) QIVLTQSPAIMSASPGEKVTMTCSASSSVSYMHWYQQKSGTSPKRWIYDTSKLASGVPARFSGSGSGTSYSLTISSMEAEDAATYYCQQWSNSPPTFGAGAKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRN |
Antigen
Chain: B
Mutation: N104Q/N108Q
| >4I9W_B|Chain A, B|Potassium channel subfamily K member 4|Homo sapiens (9606) MTTAPQEPPARPLQAGSGAGPAPGRAMRSTTLLALLALVLLYLVSGALVFRALEQPHEQQAQRELGEVREKFLRAHPCVSDQELGLLIKEVADALGGGADPETQSTSQSSHSAWDLGSAFFFSGTIITTIGYGNVALRTDAGRLFCIFYALVGIPLFGILLAGVGDRLGSSLRHGIGHIEAIFLKWHVPPELVRVLSAMLFLLIGCLLFVLTPTFVFCYMEDWSKLEAIYFVIVTLTTVGFGDYVAGADPRQDSPAYQPLVWFWILLGLAYFASVLTTIGNWLRVVSRRTRAEMGGLTAQSNSLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: SER30 TYR31 HIS33 ASP49 TRP90 SER91 ASN92 SER93 G: THR30 GLY31 TYR32 ILE33 TRP47 LEU50 ASN52 ASN54 THR55 TYR57 THR59 ASN100 TYR101 B: GLU67 ARG69 GLU70 LEU73 ARG74 PRO77 CYS78 VAL79 SER80 ASP81 GLN82 GLU83 LEU86 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
