Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6871 |
| PDBID: | 4I2X |
| Chains: | DC_F |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.48 |
| Reference: | 10.1186/1472-6807-13-13 |
| Antibody | |
| Antibody: | OX117 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Signal Regulatory Protein γ (SIRPγ) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >4I2X_D|Chain B, D|FabOX117 heavy chain|Homo sapiens (9606) EVKLQQSGPELVKPGASVKISCKASGYSFTSYYIHWVKQRPGQGLEWIGWVFPGSGNTKYNEKFKGKATLTADTSSSTAYMQLSSLTSEDSAVYFCARGNYDRAWFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCGKHHHHHH |
Light Chain: C
Mutation: NULL
| >4I2X_C|Chain A, C|FabOX117 light chain|Homo sapiens (9606) DIVITQSPKFMSTSVGDRVSITCKASQDVSTAVAWFQQKPGQSPKLLIYSASYRYTGVPDRFTGSGSGTDFTFTISSVQAEDLAVYYCQQHYSTPWTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: F
Mutation: NULL
| >4I2X_F|Chain E, F|Signal-regulatory protein gamma|Homo sapiens (9606) EEELQMIQPEKLLLVTVGKTATLHCTVTSLLPVGPVLWFRGVGPGRELIYNQKEGHFPRVTTVSDLTKRNNMDFSIRISSITPADVGTYYCVKFRKGSPENVEFKSGPGTEMALGAKPSAPVVLGPAARTTPEHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPTGQSVAYSIRSTARVVLDPWDVRSQVICEVAHVTLQGDPLRGTANLSEAIRVPPTLEVTQQPMRVGNQVNVTCQVRKFYPQSLQLTWSENGNVCQRETASTLTENKDGTYNWTSWFLVNISDQRDDVVLTCQVKHDGQLAVSKRLALEVSTRHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP1 VAL3 SER26 GLN27 SER30 ALA32 HIS91 TYR92 SER93 THR94 PRO95 TRP96 D: SER28 THR30 SER31 TYR32 TYR33 TRP50 PHE52 GLY54 SER55 ASN57 THR58 LYS59 GLU62 LYS63 LYS65 ASN100 TYR101 ASP102 ARG103 ALA104 F: GLU2 GLN5 MET6 ILE7 GLN8 PRO9 GLU10 LYS11 LEU12 LEU13 LEU14 HIS24 THR26 VAL27 THR28 VAL63 SER64 ASN71 MET72 ASP73 PRO108 GLY109 GLU111 PRO147 ARG148 ASP149 ILE150 ALA201 HIS202 VAL203 GLN206 GLY207 ASP208 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
