Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6870 |
| PDBID: | 4I18 |
| Chains: | AB_R |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.24 |
| Reference: | 10.1186/s12964-014-0080-8 |
| Antibody | |
| Antibody: | 4I18 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Prolactin receptor |
| Antigen mutation: | No |
| Durg Target: | P16471 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4I18_A|Chain B[auth A], D[auth H]|antibody heavy chain|Mus musculus (10090) EISEVQLVESGGGLVQPGGSLRLSCAASGFNFSSSSMHWVRQAPGQGLEWVAYISPYYGSTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSYGYVYWNAYSSGMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: B
Mutation: NULL
| >4I18_B|Chain A[auth B], C[auth L]|antibody light chain|Mus musculus (10090) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYSYYYPFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: R
Mutation: NULL
| >4I18_R|Chain E[auth C], F[auth R]|Prolactin receptor|Homo sapiens (9606) QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMNDT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: TYR50 TYR56 SER57 TYR95 TYR96 A: GLU4 VAL5 GLY29 PHE30 ASN31 SER34 SER35 TYR57 TYR58 SER60 ARG101 TYR103 TYR105 VAL106 TYR107 TRP108 TYR111 SER113 ASP116 TYR117 R: LYS11 ARG13 PRO15 ASN16 GLU18 THR19 THR21 TRP23 ASP53 ILE55 THR56 GLY57 GLY58 PRO59 ASN60 HIS63 GLY65 LYS66 GLN102 ASP104 PRO105 PRO106 LEU107 THR131 LEU132 ILE133 PRO196 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
