Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6865 |
| PDBID: | 4HLZ |
| Chains: | IJ_D |
| Organism: | Influenza A virus (A/Japan/305/1957(H2N2)), Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1128/JVI.02975-12 |
| Antibody | |
| Antibody: | C179 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H2N2 hemagglutinin HA2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4HLZ_I|Chain G, I, K|Fab C179 heavy chain|Mus musculus (10090) EVKLVESGGGLVQPGGSLRLSCGTSGFTLTDDYMTWVRQPPGKALEWLGFIRDRANGYTTEYSASVKGRFTISRDNSQSIVYLQMNTLRVEDSATYYCARPKGYFPYAMDYWGQGTSVIVSSTKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGHHHHHH |
Light Chain: J
Mutation: NULL
| >4HLZ_J|Chain H, J, L|Fab C179 light chain|Mus musculus (10090) DIQMTQSPASQSASLGESVTITCLASQTIGTWLAWYQQKPGKSPQLLIYAATSLADGVPSRFSGSGSGTKFSFKISSLQAEDFVSYYCQQLYSTPWTFGGGTRLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: D
Mutation: NULL
| >4HLZ_D|Chain B, D, F|Hemagglutinin HA2|Influenza A virus (387161) GLFGAIAGFIEGGWQGMVDGWYGYHHSNDQGSGYAADKESTQKAFDGITNKVNSVIEKMNTQFEAVGKEFSNLERRLENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRMQLRDNVKELGNGCFEFYHKCDDECMNSVKNGTYDYPKYEEESKLNRNEIK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: PHE27 LEU29 LYS96 GLY97 TYR98 PHE99 J: GLY32 THR33 TRP34 TYR51 ALA52 THR54 SER55 ASP58 SER69 D: VAL18 ASP19 GLY20 TRP21 ALA36 LYS38 GLU39 THR41 GLN42 LYS43 PHE45 ASP46 ILE48 THR49 ASN50 VAL52 ASN53 ILE56 ASN150 ASN154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
