Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6859 |
| PDBID: | 4HJJ |
| Chains: | HL_A |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.4161/mabs.23977 |
| Antibody | |
| Antibody: | D Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-18 (IL-18) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4HJJ_H|Chain B[auth H]|Anti-IL12 Anti-IL18 DFab Heavy Chain|Homo sapiens (9606) EVTLRESGPALVKPTQTLTLTCTFSGFSLSKSVMGVSWIRQPPGKALEWLAHIYWDDDKYYNPSLSARLTISKDTSKNQVVLTMTNMDPVDTATYYCARRGIRSAMDYWGQGTTVTVSSASTKGPEVQLVQSGTEVKKPGESLKISCKGSGYTVTSYWIGWVRQMPGKGLEWMGFIYPGDSETRYSPTFQGQVTISADKSFNTAFLQWSSLKASDTAMYYCARVGSGWYPYTFDIWGQGTMVTVSSASTKGPSVFPLAPSSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPK |
Light Chain: L
Mutation: NULL
| >4HJJ_L|Chain C[auth L]|Anti-IL12 Anti-IL18 DFab Light Chain|Homo sapiens (9606) DIVMTQSPDSLAVSLGERATINCKASQSVSNDVAWYQQKPGQPPKLLIYYASNRYTGVPDRFSGSGSGTDFTLTISSLEAEDVAVYYCQQDYNSPWTFGGGTKVEIKRTVAAPEIVMTQSPATLSVSPGERATLSCRASESISSNLAWYQQKPGQAPRLFIYTASTRATDIPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNNWPSITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: A
Mutation: C38A/C68A/C76A/C127A
| >4HJJ_A|Chain A|Interleukin-18|Homo sapiens (9606) YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDARDNAPRTIFIISMYKDSQPRGMAVTISVKAEKISTLSAENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLAAEKERDLFKLILKKEDELGDRSIMFTVQNEA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLY124 PRO125 GLU126 VAL127 GLY151 TYR152 THR153 THR155 SER156 TYR157 TRP158 TYR177 GLY179 ASP180 GLU182 ARG184 LYS199 ARG223 VAL224 GLY225 SER226 GLY227 TRP228 TYR229 PRO230 TYR231 ASP234 L: TYR162 THR163 THR166 ALA168 THR169 TRP207 PRO208 A: GLN60 GLY61 ASN62 ARG63 PRO64 LEU65 GLU113 ASN114 LYS115 ILE116 ILE117 SER118 TYR159 GLU164 LYS165 GLU166 ARG167 LEU169 LYS171 LEU172 ILE173 LEU174 LYS175 LYS176 LEU180 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
