Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6858 |
| PDBID: | 4HJG |
| Chains: | BA_E |
| Organism: | Homo sapiens, Staphylococcus aureus subsp. aureus Mu50, Finegoldia magna |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1073/pnas.1307309110 |
| Antibody | |
| Antibody: | Trastuzumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Fab binding protein L |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >4HJG_B|Chain B|Trastuzumab heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQSPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAIYYCSRWGGDGFYAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Light Chain: A
Mutation: NULL
| >4HJG_A|Chain A|Trastuzumab light chain|Homo sapiens (9606) DIQMTQSPILLSASVGDRVTITCRASQDVNTAVAWYQQRTNGSPRLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDIADYYCQQHYTTPPTFGAGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: NULL
| >4HJG_E|Chain D[auth E]|Protein L fragment|Finegoldia magna (1260) SGSEVTIKVNLIFADGKIQTAEFKGTFEEATAEAYRYAALLAKVNGEYTADLEDGGNHMNIKFAG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: THR5 SER7 PRO8 ILE9 LEU10 LEU11 SER12 ALA13 SER14 GLY16 ASP17 ARG18 VAL19 THR20 THR22 ARG24 SER67 ASP70 THR72 LYS107 E: LEU27 PHE29 LYS33 ILE34 GLN35 THR36 ALA37 GLU38 PHE39 LYS40 GLU49 ARG52 TYR53 LEU56 LEU57 LYS59 VAL60 ASN61 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
