Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6855 |
| PDBID: | 4HJ0 |
| Chains: | CD_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1074/jbc.M112.426288 |
| Antibody | |
| Antibody: | Gipg013 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glucose-dependent insulinotropic polypeptide receptor (GIPr) ECD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >4HJ0_C|Chain C, F[auth P]|Gipg013 Fab, Antagonizing antibody to the GIP Receptor, Heavy chain|Homo sapiens (9606) QVQLQQSGAEVKKPGSSVKVSCKASGGTFSSYAISWVRQAPGQGLEWMGGIIPTFGTANYAQKFQGRVTITADESTSTAYMELSSLRSEDTAVYYCAQGPIVGAPTDYWGKGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTHT |
Light Chain: D
Mutation: NULL
| >4HJ0_D|Chain D, E[auth Q]|Gipg013 Fab, Antagonizing antibody to the GIP Receptor, Light chain|Homo sapiens (9606) SYVLTQPPSASGTPGQRVAISCSGSNSNIGSNTVHWYQQLPGAAPKLLIYSNNQRPSGVPDRFSGSNSGTSASLAISRLQSEDEADYYCAAWDDSLNGVVFGGGTKVTVLQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: B
Mutation: NULL
| >4HJ0_B|Chain A, B|Gastric inhibitory polypeptide receptor|Homo sapiens (9606) MGSSHHHHHHSDYKDDDDKHMETGSKGQTAGELYQRWERYRRECQETLAAAEPPSGLACNGSFDMYVCWDYAAPNATARASCPWYLPWHHHVAAGFVLRQCGSDGQWGLWRDHTQCENPEKNEAFLDQRLILERLQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: ASN32 THR33 HIS35 LEU47 TYR50 SER51 GLN54 ARG55 PRO56 SER57 TRP92 C: GLY26 GLY27 THR28 SER30 SER31 TYR32 ILE52 THR54 PHE55 ASN59 GLN98 GLY99 PRO100 ILE101 VAL102 GLY103 ALA104 PRO105 ASP107 TYR108 B: ALA32 GLY33 LEU35 TYR36 GLN37 TRP39 GLU40 ARG43 GLN47 PHE65 MET67 TYR68 VAL69 TYR87 LEU88 PRO89 TRP90 TRP109 ARG113 ASP114 HIS115 THR116 CYS118 GLU119 ASN120 PRO121 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
