Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6845 |
| PDBID: | 4HFU |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/Japan/305+/1957(H2N2)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.11 |
| Reference: | 10.1038/nsmb.2500 |
| Antibody | |
| Antibody: | 8M2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H2N2 hemagglutinin HA1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4HFU_H|Chain C[auth H]|Fab 8M2 heavy chain|Homo sapiens (9606) EVQLVESGADMKPPGSSVKVPCKASGDTFSSYTITWVRQAPGQGLEWMGGITPIFGSPNYAQRFQDRVIITADESTSTAYMEVSNLRSEDTAVYFCARVGGEWGSGRYYLDHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: L
Mutation: NULL
| >4HFU_L|Chain D[auth L]|Fab 8M2 light chain|Homo sapiens (9606) DIQLTQSPASLSVSPGERATLSCRASQSVAGNLAWYQQKPGQAPRLLIYGASTRATGIPARFSGSGSGTEFTLTITSLQSEDFAVYYCQQYNNWPPWTFGQGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >4HFU_A|Chain A|Hemagglutinin HA1|Influenza A virus (382813) PGDQICIGYHANNSTEKVDTILERNVTVTHAKDILEKTHNGKLCKLNGIPPLELGDCSIAGWLLGNPECDRLLSVPEWSYIMEKENPRDGLCYPGSFNDYEELKYLLSSVKHFEKVKILPKDRWTQHTTTGGSRACAVSGNPSFFRNMVWLTKKGSDYPVAKGSYNNTSGEQMLIIWGVHHPNDETEQRTLYQNVGTYVSVGTSTLNKRSTPEIATRPKVNGLGSRMEFSWTLLDMWDTINFESTGNLIAPEYGFKISKRGSSGIMKTEGTLENCETKCQTPLGAINTTLPFHNVHPLTIGECPRYVKSEKLVLATGLRNVPQIESR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 PHE29 SER31 TYR32 ILE53 PHE54 SER56 PRO57 ASN58 TYR59 GLN64 GLU73 GLU98 TRP99 L: ASN92 ASN93 TRP94 PRO95 TRP96 A: TYR98 THR131 THR133 GLY134 GLY135 SER136 ARG137 ALA138 GLY143 ASN144 PRO145 TRP153 THR155 LYS156 LYS157 GLY158 SER159 ASN186 ASP187 GLU188 THR189 GLU190 ARG192 THR193 LEU194 LYS222 GLY225 LEU226 GLY227 SER228 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
