Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6844 |
| PDBID: | 4HF5 |
| Chains: | HL_A |
| Organism: | Influenza A virus (A/Japan/305+/1957(H2N2)), Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1038/nsmb.2500 |
| Antibody | |
| Antibody: | 8F8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus H2N2 hemagglutinin HA1 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4HF5_H|Chain C[auth H]|Fab 8F8 heavy chain|Homo sapiens (9606) EVQLVESGGGVVQPGRSLRLSCAASGFMFSSYVMHWVRQPPGKGLEWVAVIWYDGSKTYFADSMRGRLTVSRDNSKNALYLQMNRLRAEDTAVYYCARQQDSGYSGPEVSYYSHYGMDVWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: L
Mutation: NULL
| >4HF5_L|Chain D[auth L]|Fab 8F8 light chain|Homo sapiens (9606) QSVLTQSPSASGTPGQAITISCSGSSSNIGSNPVNWYQQLPGAAPKLLIYADEHRPSGVPDRFSGSKSGTSASLAISGLQSEDEADYYCAAWDDSLSGPAVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >4HF5_A|Chain A|Hemagglutinin HA1|Influenza A virus (382813) PGDQICIGYHANNSTEKVDTILERNVTVTHAKDILEKTHNGKLCKLNGIPPLELGDCSIAGWLLGNPECDRLLSVPEWSYIMEKENPRDGLCYPGSFNDYEELKHLLSSVKHFEKVKILPKDRWTQHTTTGGSRACAVSGNPSFFRNMVWLTEKGSNYPVAKGSYNNTSGEQMLIIWGVHHPNDETEQRTLYQNVGTYVSVGTSTLNKRSTPEIATRPKVNGQGGRMEFSWTLLDMWDTINFESTGNLIAPEYGFKISKRGSSGIMKTEGTLENCETKCQTPLGAINTTLPFHNVHPLTIGECPKYVKSEKLVLATGLRNVPQIESR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: MET28 SER30 SER31 TYR32 TYR52 ASP53 ASN73 ASP97 SER98 GLY99 L: TYR49 ALA50 HIS53 A: TYR98 THR131 THR133 GLY134 GLY135 SER136 ARG137 ALA140 GLY143 ASN144 PRO145 TRP153 THR155 GLU156 LYS157 GLY158 SER159 ASP187 GLU188 THR189 GLU190 ARG192 THR193 LEU194 GLY225 GLN226 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
