Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6834 |
| PDBID: | 4HC1 |
| Chains: | HL_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.87 |
| Reference: | Not available or To be published |
| Antibody | |
| Antibody: | 10G3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4HC1_H|Chain B[auth H], E[auth M]|10G3 heavy chain|Mus musculus (10090) DVQLQESGPGLVKPSQSLSLTCSVTGYSITSGYYWNWIRQFPGNKLEWMGYISYDGSNNYNPSLKNRVSITRDTSKNHFFLKLSSVTTEDTATYYCARASDSDGFAYWGQGTLVTVSAAKTTAPSVYPLAPVCGGTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAILQSGLYTLSSSVTVTSNTWPSQTITCNVAHPASSTKVDKKIEPRVP |
Light Chain: L
Mutation: NULL
| >4HC1_L|Chain C[auth L], F[auth N]|10G3 light chain|Mus musculus (10090) DILMTQSPSSMSVSLGDTVSFTCHASQGIGRNIGWLQQKPGKSFKGLIYHGTNLKDGVPSRFSGSGSGADYSLTISRIESEDFADYYCIQYVQFPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: D94N/E148Q
| >4HC1_A|Chain A, D[auth B]|Mucosal addressin cell adhesion molecule 1|Homo sapiens (9606) VKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPNQLTVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEVQQEPIGGDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHRQAIPVLGGENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: TYR27 SER31 GLY32 TYR33 TYR34 TYR51 TYR54 ALA99 SER100 ASP101 SER102 PHE105 L: ILE29 GLY30 ARG31 ASN32 HIS50 THR52 ASN53 SER67 GLY68 TYR91 VAL92 TYR96 A: ALA34 SER35 GLN37 GLY40 LEU41 ASP42 THR43 SER44 LEU45 GLY46 ALA47 VAL48 GLN49 SER50 ASP51 THR52 SER63 SER65 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
