Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6832 |
| PDBID: | 4GRW |
| Chains: | G_B |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.3389/fimmu.2017.00884 |
| Antibody | |
| Antibody: | 124C4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-23 subunit beta (IL-23 β) |
| Antigen mutation: | No |
| Durg Target: | P29460; P29460; |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >4GRW_G|Chain E, G|Nanobody 124C4|Lama glama (9844) EVQLVESGGGLVQPGGSLRLSCAASGFTLDDYAIAWFRQAPGKEREGVSGIDSGDGSAYYADSVKGRFTISSDNAKNTVYLQMNSLRPEDTAVYYCARVRTGWGLNAPDYAMDYWGKGTLVTVSS |
Antigen
Chain: B
Mutation: NULL
| >4GRW_B|Chain B, D|Interleukin-12 subunit beta|Homo sapiens (9606) MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| G: TYR28 ALA29 ASP48 GLY50 ASP51 GLY52 SER53 ALA54 TYR55 VAL95 THR97 GLY98 TRP99 GLY100 LEU101 ASN102 PRO104 TYR106 B: ASN113 SER115 ARG117 GLN172 PRO178 ALA179 LYS217 GLU235 TYR236 ASP238 THR239 TRP240 SER241 THR242 PRO243 HIS244 SER245 PHE247 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
