Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6829 |
| PDBID: | 4GRW |
| Chains: | I_D |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.3389/fimmu.2017.00884 |
| Antibody | |
| Antibody: | 22E11 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-23 subunit beta (IL-23 β) |
| Antigen mutation: | No |
| Durg Target: | P29460; P29460; |
Sequence information
Antibody
Chain: I
Mutation: NULL
| >4GRW_I|Chain H, I|Nanobody 22E11|Lama glama (9844) EVQLVESGGGLVQAGGSLRLSCAASGRTFSWSAVGWFRQAPGKEREFVAAIRWSGGSPYYADSVKDRFTISRDNAKNTVYLQMNSLRPEDTAVYLCGETSLFPTSRGSHYDTWGQGTQVTVSS |
Antigen
Chain: D
Mutation: NULL
| >4GRW_D|Chain B, D|Interleukin-12 subunit beta|Homo sapiens (9606) MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition I: ARG27 THR28 PHE29 SER30 TRP31 GLU44 ARG52 GLY56 SER57 PRO58 TYR59 ASP62 LYS65 SER100 LEU101 PHE102 PRO103 THR104 SER105 ARG106 HIS109 ASP111 D: LEU13 TRP15 PRO17 ASP18 LEU40 ASP41 GLU45 LYS58 GLU59 PHE60 GLY61 ALA63 LYS84 GLU86 ASP93 THR125 ILE126 SER127 THR128 ASN162 HIS194 LYS195 LEU196 LYS197 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
