Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6828 |
| PDBID: | 4GRW |
| Chains: | E_D |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.55 |
| Reference: | 10.3389/fimmu.2017.00884 |
| Antibody | |
| Antibody: | 124C4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-23 subunit beta (IL-23 β) |
| Antigen mutation: | No |
| Durg Target: | P29460; P29460; |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >4GRW_E|Chain E, G|Nanobody 124C4|Lama glama (9844) EVQLVESGGGLVQPGGSLRLSCAASGFTLDDYAIAWFRQAPGKEREGVSGIDSGDGSAYYADSVKGRFTISSDNAKNTVYLQMNSLRPEDTAVYYCARVRTGWGLNAPDYAMDYWGKGTLVTVSS |
Antigen
Chain: D
Mutation: NULL
| >4GRW_D|Chain B, D|Interleukin-12 subunit beta|Homo sapiens (9606) MCHQQLVISWFSLVFLASPLVAIWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ASP30 ASP31 TYR32 ALA33 ASP52 GLY54 ASP55 SER57 ALA58 TYR59 TYR60 VAL99 THR101 GLY102 TRP103 GLY104 LEU105 ASN106 TYR110 D: ASN113 SER115 ARG117 GLN172 PRO178 ALA179 LYS217 GLU235 TYR236 ASP238 THR239 TRP240 SER241 THR242 PRO243 HIS244 SER245 PHE247 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
