Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 682 |
| PDBID: | 3V6O |
| Chains: | CE_A |
| Organism: | Homo sapiens, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 1.95 |
| Reference: | 10.1016/j.str.2012.01.019 |
| Antibody | |
| Antibody: | 9F8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Leptin receptor |
| Antigen mutation: | No |
| Durg Target: | P48357 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >3V6O_C|Chain C, D|Monoclonal antibody 9F8 fab fragment Heavy chain|Mus musculus (10090) AEVKLLESGPGLVAPSESLSITCTISGFSLTDDGVSWIRQPPGKGLEWLGVIWGGGSTYFNSLFKSRLSITRDNSKSQVFLEMDSLQTDDTAMYYCAKHDGHETMDYWGQGTSVTVSSSKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDCT |
Light Chain: E
Mutation: NULL
| >3V6O_E|Chain E, F|Monoclonal antibody 9F8 fab fragment Light chain|Mus musculus (10090) AEIVMTQSPKFMSTSIGDRVNITCKATQNVRTAVTWYQQKPGQSPQALIFLASNRHTGVPARFTGSGSGTDFTLTINNVKSEDLADYFCLQHWNYPLTFGSGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >3V6O_A|Chain A, B|Leptin receptor|Homo sapiens (9606) IDVNINISCETDGYLTKMTCRWSTSTIQSLAESTLQLRYHRSSLYCSDIPSIHPISEPKDCYLQSDGFYECIFQPIFLLSGYTMWIRINHSLGSLDSPPTCVLPDSVVKPLPPSSVKAEITINIGLLKISWEKPVFPENNLQFQIRYGLSGKEVQWKMYEVYDAKSKSVSLPVPDLCAVYAVQVRCKRLDGLGYWSNWSNPAYTVV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: ASP33 ASP34 TRP54 GLY55 GLY56 SER58 TYR60 ASP101 GLY102 HIS103 GLU104 THR105 E: PHE51 LEU52 ASN55 HIS93 TRP94 ASN95 TYR96 LEU98 A: ARG465 TYR466 HIS467 ARG468 SER469 SER470 LEU471 SER478 ILE479 HIS480 PRO481 ILE482 SER483 GLU484 PRO485 PRO502 PHE504 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
