Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6813 |
| PDBID: | 4FFY |
| Chains: | HL_A |
| Organism: | Mus musculus, dengue virus type 1 |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1371/journal.ppat.1002930 |
| Antibody | |
| Antibody: | DENV1-E111 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dengue virus (DENV) type 1 envelope glycoprotein domain III |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4FFY_H|Chain B[auth H]|DENV1-E111 single chain variable fragment (heavy chain)|Mus musculus (10090) QVQLLQPGAELVKPGASMKLSCKASGYTFTNWWMHWVRLRPGRGLEWIGRIDPNSDVNKYNEKFENRASLTVDKHSSTAYMQLSSLTSEDSAIYYCARWFFPWYFDVWGTGTTVTVSSAASGADHHHHHH |
Light Chain: L
Mutation: NULL
| >4FFY_L|Chain A[auth L]|DENV1-E111 single chain variable fragment (light chain)|Mus musculus (10090) NIVLTQSPASLAVSLGQRATISCRASESVDHYGNSFIYWYQQKPGQPPKLLIYLASNLESGVPARFSGSGSETDFTLTIDSVETDDAATYYCQQNNEDPYTFGGGTKLEIKGGGGSGGGGSGGGGS |
Antigen
Chain: A
Mutation: NULL
| >4FFY_A|Chain C[auth A]|envelope glycoprotein|Dengue virus 1 (11053) MASMTLKGMSYVMCTGSFKLEKEVAETQHGTVLVQVKYEGTDAPCKIPFSTQDEKGATQNGRLITANPIVTDKEKPVNIEAEPPFGESYIVVGAGEKALKLSWFKKGSSIG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: ASN30 PHE32 TYR49 LEU50 ASN53 ASN91 ASN92 GLU93 ASP94 TYR96 H: ASN31 TRP32 TRP33 ARG50 ASP52 VAL57 ASN58 LYS59 TRP99 PHE100 PHE101 PRO102 TRP103 A: TYR299 VAL300 MET301 CYS302 THR303 LYS334 ILE335 PRO336 PHE337 SER338 THR339 GLN340 GLU342 LYS343 GLY344 ALA345 THR346 GLN347 ASN348 GLY349 ARG350 LEU351 ASN355 ALA369 GLU370 PRO371 VAL379 GLY381 ALA382 GLY383 GLU384 ALA386 LYS388 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
