Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6806 |
| PDBID: | 4F37 |
| Chains: | HL_A |
| Organism: | Escherichia coli, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.57 |
| Reference: | 10.1002/prot.24312 |
| Antibody | |
| Antibody: | WO2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Aβ residues 1-16 fused to the N-terminus of the Escherichia coli immunity protein Im7 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >4F37_H|Chain C[auth F], D[auth H]|Im7 immunity protein|Mus musculus (10090) QVTLKESGPGILQPSQTLSLTCSFSGFSLRTSRVGVSWIRQPSGKGLEWLAHIYWDDDKRYNPSLESRLTISKDTSRNQVFLKITSVDTADTATYYCARRGFYGRKYEVNHFDYWGQGTTLTVSSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSESITCNVAHPASSTKVDKKIVPRDCG |
Light Chain: L
Mutation: NULL
| >4F37_L|Chain E[auth K], F[auth L]|Fab WO2 anti-amyloid-beta antibody Fab fragment|Mus musculus (10090) DIVMTQTPLSLPVSLGDQASISCRSSQTILHSNGNTYLEWYLQKPGQSPNLLIYKVSKRFSGVPDRFSGSGSGTDFTLKISRVEAEDLGVYYCFQGSRVPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: A
Mutation: NULL
| >4F37_A|Chain A, B|Colicin-E7 immunity protein|Escherichia coli (562) DAEFRHDSGYEVHHQKSELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKITEHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQGAAADYKDDDDKAADYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ARG33 TRP49 HIS52 TYR54 TRP55 ASP56 ASP58 ARG60 PRO63 ARG100 ARG105 TYR107 GLU108 ASN110 L: HIS31 SER32 ASN33 ASN35 TYR37 GLY96 SER97 ARG98 VAL99 LEU101 A: ASP1 ALA2 GLU3 PHE4 ARG5 HIS6 ASP7 SER8 GLY9 TYR10 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
