Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6798 |
| PDBID: | 4EIZ |
| Chains: | D_A |
| Organism: | Escherichia coli K-12, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1016/j.bbapap.2013.07.010 |
| Antibody | |
| Antibody: | 113 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Dihydrofolate reductase (DHFR) |
| Antigen mutation: | No |
| Durg Target: | P0ABQ4 |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >4EIZ_D|Chain C, D|Nb113 Camel antibody fragment|Lama glama (9844) QVQLQESGGGLVQAGGSLRLSCTASGRTFSSYAMGWFRQTPGKEREFVAAITWGGSTTLYADSVKGRFTMSRDNAKNTVYLQMNSLKPEDTAVYYCAADGSQYRSTYSFRDKPDYGSWGQGTQVTVSSHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >4EIZ_A|Chain A, B|Dihydrofolate reductase|Escherichia coli (83333) MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| D: SER30 THR52 TRP53 GLY54 SER56 THR57 ASN74 GLN102 TYR103 ARG104 SER105 THR106 TYR107 SER108 A: VAL10 ASP11 HIS114 ILE115 ASP116 ALA117 GLU118 TYR128 SER138 PHE140 ASP142 HIS149 SER150 TYR151 CYS152 GLU154 LEU156 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
