Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6792 |
| PDBID: | 4DKF |
| Chains: | IM_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.61 |
| Reference: | 10.1016/j.str.2012.02.010 |
| Antibody | |
| Antibody: | 2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-34 (IL-34) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4DKF_I|Chain C[auth H], E[auth I]|FAb2 Heavy Chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFSFTSYGISWVRQAPGKGLEWVSHIDWYGGDTDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGGPDYAMDVWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: M
Mutation: NULL
| >4DKF_M|Chain D[auth L], F[auth M]|FAb2 Light Chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQSISSYLAWYQQKPGKAPKLLIYGASSRASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYWSEPVTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >4DKF_B|Chain A, B|Interleukin-34|Homo sapiens (9606) AGSNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVGNSGNSDYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: THR30 SER31 TRP52 TYR53 GLY54 GLY55 ASP56 PRO97 ASP98 M: SER30 TYR32 TRP92 SER93 GLU94 B: TYR47 ARG50 LEU51 LYS55 ILE60 ASN61 LYS63 ARG73 ILE74 ALA75 LYS157 LEU158 ARG160 LYS162 ALA163 ASP166 ASN167 ARG170 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
