Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6790 |
| PDBID: | 4DKE |
| Chains: | IM_B |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1016/j.str.2012.02.010 |
| Antibody | |
| Antibody: | 1.1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Interleukin-34 (IL-34) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: I
Mutation: NULL
| >4DKE_I|Chain C[auth H], E[auth I]|FAb1.1 Heavy Chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFTFSSTWIHWVRQAPGKGLEWVARISPYYYYSDYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGLGKGSKRGAMDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: M
Mutation: NULL
| >4DKE_M|Chain D[auth L], F[auth M]|FAb1.1 Light Chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVSTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQSFYFPNTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >4DKE_B|Chain A, B|Interleukin-34|Homo sapiens (9606) AGSNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVGNSGNSDYKDDDDK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| I: TRP33 ARG50 TYR53 TYR54 TYR56 LYS98 GLY99 M: ASP28 SER30 THR31 ALA32 PHE53 SER91 PHE92 TYR93 PHE94 ASN96 B: SER65 GLU103 SER104 GLN106 ASP107 VAL108 LEU109 LEU110 GLU111 GLY112 TRP116 LYS117 GLN120 GLU121 GLU123 THR124 LEU127 ASN155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
