Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6783 |
| PDBID: | 4CNI |
| Chains: | AB_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.4161/mabs.28612 |
| Antibody | |
| Antibody: | Olokizumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Olokizumab(Approved) |
| Antigen | |
| Antigen: | Interleukin-6 (IL-6) |
| Antigen mutation: | No |
| Durg Target: | P05231 |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >4CNI_A|Chain A, E[auth H]|OLOKIZUMAB HEAVY CHAIN, FAB PORTION|HOMO SAPIENS (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNFNDYFMNWVRQAPGKGLEWVAQMRNKNYQYGTYYAESLEGRFTISRDDSKNSLYLQMNSLKTEDTAVYYCARESYYGFTSYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVES |
Light Chain: B
Mutation: NULL
| >4CNI_B|Chain B, F[auth L]|OLOKIZUMAB LIGHT CHAIN, FAB PORTION|HOMO SAPIENS (9606) DIQMTQSPSSLSASVGDRVTITCQASQDIGISLSWYQQKPGKAPKLLIYNANNLADGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCLQHNSAPYTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: NULL
| >4CNI_D|Chain C, D|INTERLEUKIN-6|HOMO SAPIENS (9606) PHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ASP31 PHE33 ARG52 ASN53 ASN55 TYR56 GLN57 TYR61 GLU101 SER102 TYR103 TYR104 GLY105 B: GLN27 ASP28 GLY30 ILE31 SER32 ASN50 SER67 HIS91 ASN92 SER93 ALA94 TYR96 D: SER47 ASN48 LYS54 LEU57 ASN60 ASN103 ARG104 GLU106 THR149 LYS150 GLN152 ALA153 GLN154 ASN155 GLN156 TRP157 LEU158 GLN159 ASP160 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
