Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 6781 |
| PDBID: | 4CAD |
| Chains: | BA_C |
| Organism: | Mus musculus, Methanococcus maripaludis |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1038/nature12754 |
| Antibody | |
| Antibody: | 645-2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Rce1 from the archaea Methanococcus maripaludis (MmRce1) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >4CAD_B|Chain B, E, H, K|ANTIBODY FAB FRAGMENT HEAVY CHAIN|MUS MUSCULUS (10090) QVQLKQSGAELMKPGASVKISCKATGYKFSSYWIEWVKQRPGHGLEWIGEIFPGSGNTNYNEKFKGKATLTADTSSNTAYMQLSSLTSEDSAVYYCARRGAFYSYGSSYYAMDFWGQGTSVTVSSAKTTPPSDYPLAPVCGDTSGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGP |
Light Chain: A
Mutation: NULL
| >4CAD_A|Chain A, D, G, J|ANTIBODY FAB FRAGMENT LIGHT CHAIN|MUS MUSCULUS (10090) DIQLTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYNAKTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGNYYCQHFWSTPWTFGGGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: C
Mutation: NULL
| >4CAD_C|Chain C, F, I, L|RAS AND A-FACTOR CONVERTING ENZYME 1, RCE1|METHANOCOCCUS MARIPALUDIS (39152) MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIAKSKNNDLKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGFSFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVNNSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAITQDTKIIETGVLFLVAAAIILYDKKMFFEKLG |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: ILE2 SER26 GLY27 ASN28 HIS30 TYR32 PHE91 TRP92 SER93 TRP96 B: LYS28 SER30 SER31 TRP33 PHE52 SER55 ASN57 ASN59 ARG99 ALA101 PHE102 TYR103 SER104 TYR105 GLY106 TYR109 TYR110 C: SER31 PHE32 SER33 SER34 SER37 GLY38 TYR40 GLY108 GLY109 GLN113 SER117 GLY118 GLY119 ASP125 PHE126 VAL129 ASN180 ASN181 TYR183 GLU186 ILE187 PHE188 ASN189 GLN190 SER191 ILE192 TYR194 GLN234 GLU235 LEU236 ALA238 ILE239 THR240 GLN241 ASP242 LYS244 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
