Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 678 |
| PDBID: | 3UZQ |
| Chains: | A_B |
| Organism: | Mus musculus, dengue virus type 1 |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.1016/j.str.2012.01.001 |
| Antibody | |
| Antibody: | 4E11 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | dengue virus serotype 1 envelope protein domain III |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >3UZQ_A|Chain A|anti-dengue Mab 4E11|Mus musculus (10090) EVKLLEQSGAELVKPGASVRLSCTASGFNIKDTYMSWVKQRPEQGLEWIGRIDPANGDTKYDPKFQGKATITADTSSNTAYLHLSSLTSGDTAVYYCSRGWEGFAYWGQGTLVTVSAGGGGSGGGGSGGGGSELVMTQTPASLAVSLGQRATISCRASENVDRYGNSFMHWYQQKAGQPPKLLIYRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYFCQRSNEVPWTFGGGTKLEIKRPLEHHHHHH |
Antigen
Chain: B
Mutation: NULL
| >3UZQ_B|Chain B|envelope protein|Dengue virus 1 (11053) MGMSYVMCTGSFKLEKEVAETQHGTVLVQVKYEGTDAPCKIPFSTQDEKGVTQNGRLITANPIVTDKEKPVNIETEPPFGESYIIVGAGEKALKLSWFKKGSSIGKLEHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition A: LEU4 GLY27 PHE28 ASN29 LYS31 ASP32 THR33 TYR34 ARG51 ASP53 ALA55 ARG99 GLY100 TRP101 GLU102 ALA105 TYR106 ARG163 TYR164 GLY165 ASN166 PHE168 LEU182 TYR185 ARG186 ASN189 GLU191 SER192 GLU229 B: SER305 PHE306 LYS307 LEU308 GLU309 LYS310 GLU311 VAL312 GLN323 LYS325 GLU327 ASP360 LYS361 GLU362 LYS363 PRO364 LYS385 LEU387 LYS388 LEU389 SER390 TRP391 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
